cytometer Options
cytometer Options
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Information-enriched characterization of cell populations. Morphological info from visuals can incorporate on the richness of flow cytometry data. As an example, the figure demonstrates an usually typical apoptosis assay working with Annexin V and PI, introducing mobile imaging to characterize cells in Every populace to expose morphologically distinct features. These insights couldn't are actually received from multiplex staining by yourself.
Irrespective of whether you’re a starter or a specialist, explore our means to progress your investigation and abilities in flow cytometry.
Merge acoustic concentrating technological know-how with significant-parameter Investigation for versatile spectral unmixing and traditional workflows.
The award-winning Invitrogen Attune CytPix Flow Cytometer combines acoustic concentrating which has a high-speed brightfield camera for simultaneous large throughput flow cytometry and large resolution brightfield imaging.
"A picture isn't very well worth a thousand dots, however it genuinely helps you to see pictures. It is great to check out illustrations or photos that correlate with staining. As an example, AnnexinV-positive cells that are morphologically unique than healthful cells, or CD14+ monocytes which are larger sized and much more textured than CD3+/CD4+/CD14- lymphocytes.
Furthermore, it truly is illuminating to view the amount of doublets slip as a result of conventional singlet gates, and terrific to validate the position of surprising double-favourable situations in my Examination."
Optimizing gating method. Even strong manual singlet gating is mistake-prone and stays a subjective final decision position in Nearly all flow cytometry assays. Imaging can be utilized to verify and alter gates to include only one cells of fascination.
Our scientists will design panels for you as often times you require—cost-free, no product order desired.
Early signs of this contamination had to begin with been dismissed as particles. Backgating to find out new populations. Backgating imaged cells around the Attune CytPix Flow Cytometer also means that you can use morphological capabilities to find out intriguing subpopulations that will not be evident from flow cytometry info on your own.
Customers can perspective function illustrations or photos in genuine-time to substantiate sample excellent, enhance protocols, and file the morphology of each mobile inhabitants recognized for analysis. Imaging capture also Rewards from acoustic focusing, which helps you to place the cells to ensure that a sharp, centered image is received.
Seem waves empower specific cell alignment, resulting in exceptional details quality and ~10X more quickly sample flow rates.
We Formerly shown the strength of imaging Automobile-T/Ramos cell interactions. Allow’s have a look at just the inhabitants of finest interest, the double good gatherings, To find out more. We can now use extended morphological parameters (right here, circularity and skewness of depth) to further more analyze the features of such populations and refine gating on these activities, raising knowledge robustness.
Leverage the strength of advanced engineering and innovation to accelerate your discoveries. Rely on in the Attune Flow Cytometer spouse and children to deliver the trustworthiness, more info pace, and accuracy that set your do the job aside.
No wash/no lyse leukocyte Investigation with imaging. Cells were obtained from 24-hour-outdated blood by dilution in 1 mM EDTA ( Specialist UX/UI permits customers to prepare AI designs for expanded apps. (A) The “Coach Product” characteristic gives annotation instruments and serious-time design instruction precision.
At the heart of each and every Attune Flow Cytometer lies our revolutionary acoustic focusing technological innovation, which makes use of audio waves to meticulously align cells inside the flow cell. This impressive tactic surpasses regular hydrodynamic approaches, resulting in more rapidly processing speeds, Outstanding information high-quality, and greater sample throughput.
The Attune Xenith cytometer is really a high-parameter spectral analyzer, furnishing the two spectral unmixing and conventional flow abilities for thorough and multipurpose cell analysis.
Agilent Benefit Promise ensures you at the very least a decade of instrument use with the date you buy. Or else, We're going to credit rating you with the residual price of the system towards an upgraded design.
What seemed to be a homogeneous population is really more assorted—an insight that needs to be thought of when interpreting results.